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Fractionner une cellule diagonalement dans un tableau Word
Citing the Back Cover of a Book. Two Footnotes in a Single Sentence. Answer Ideally, it is better not to include two footnotes or endnotes in a same sentence. In this example we would like to find the concentration of target protein Y value by evaluating the curve fit at a given OD X value. The calculator will then show the corresponding y value.
Press [ Info ] button to open model information dialog which gives all of the information needed to apply a curve fit and some supplemental information about the performance of the given model.
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The most important information contained in this panel is the values of the parameters of the model. The Model section gives the data modeling equation so that the interpretation of the coefficients is clear.
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The coefficients a, b, c, etc. The Coefficients section gives the values of the relevant parameters for the current model -- these coefficients are always expressed as a, b, c, d, and so on. These coefficients match the model shown in the Model section. If the list of coefficients exceeds the extent of the window that they are shown in, a scrollbar will appear to allow you to scroll through the parameters. If we click [ Copy ], the list of coefficients is copied to the clipboard.
Then paste them in any Excel sheet.
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Bring X OD , a, b, c, d value into the formula respectively and then press [ Enter ] on your keyboard to calculate concentration. To get the most accurate results, it is recommended to do a pre-text to determine the appropriate dilution factor to make sure that the concentration of target protein in the samples after dilution falls into the range of standard curve.
If the sample has been diluted, the dilution factor should be taken in to account during calculation.
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Therefore, you should run standards in each assay to avoid systematic deviation caused by difference on operator, pipetting, incubations, and temperature of each assay. Please download Curve Expert1. If you need the software for Macs, you can download here: It is a paid software.
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If you are not willing to use it, you can make the standard curve by Excel or other softwares, or you can send us your data and we will process the data for you. Join the 25, subscribers to get research hotpots, technical tips, latest information on events, sales and offers.
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Protocols References Download Center. Technical Articles A collection of articles that focus on an array of different scientific topics such as pathways, cancer, transmembrane proteins. Distributors Worldwide Contact Us. You can also watch this video at first. Part 1: You can see an interface below. Choose the best fitting curve 2.
Calculate the concentration of target protein by interpolation How to calculate concentration? Model The Model section gives the data modeling equation so that the interpretation of the coefficients is clear. Coefficients The Coefficients section gives the values of the relevant parameters for the current model -- these coefficients are always expressed as a, b, c, d, and so on.
Notes Please download Curve Expert1.
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